Qualitative and Quantitative Study of Phytochemicals and Antioxidant Activity of Ethanolic Extract of Notonia Grandiflora Leaves

Abstract

The present study aimed to evaluate the phytochemical composition and antioxidant activity of the ethanolic extract of Notonia grandiflora leaves. The extraction process yielded 12.5% ethanolic extract, indicating a high content of polar phytoconstituents. Preliminary phytochemical screening confirmed the presence of important bioactive compounds such as alkaloids, flavonoids, phenols, glycosides, saponins, tannins, and proteins. Quantitative analysis revealed that the extract contained 0.52 mg/100 mg of total flavonoids and 0.89 mg/100 mg of total phenols, which are known contributors to antioxidant activity. The antioxidant potential was assessed using the DPPH radical scavenging assay. The ethanolic extract showed dose-dependent inhibition of DPPH radicals with a maximum of 51.87% at 100 µg/ml, and an IC₅₀ value of 95.11 µg/ml compared to 18.14 µg/ml for the standard antioxidant, ascorbic acid. These findings suggest that Notonia grandiflora leaves are a rich source of antioxidant phytochemicals and may have potential applications in the development of natural antioxidant therapies.