A REVIEW ON TROUBLE SHOOTING IN HPLC AND ITS PREVENTIVE MEASURES

Abstract

High Performance Liquid Chromatography provide much higher resolution, more accurate quantitative results, as well as shorter analysis times in comparison to the earlier techniques. HPLC has evolved into an indispensable tool in many analytical laboratories and is applied to diverse analytical problems. Trouble shooting HPLC instrumentation and separations require a fundamental understanding of how the instrument functions and how the separation works. Common HPLC problems are caused by component malfunctions like pump, degasser, injector, data system, column, and faulty preparation of the mobile phase or sample preparation. HPLC is a cornerstone technique across diverse scientific domain, yet its effectiveness can be compromised by issues ranging from peak broadening to baseline instability. It navigates through critical aspects such as column selection, mobile phase optimization, sample preparation techniques, and instrument maintenance protocols. Main key to resolve HPLC problem is cleaning of HPLC with appropriate solvent. Best approach for trouble shooting HPLC problems is going with systematic way. At the start of quantitative chromatographic analysis, the first parameter of paramount importance is baseline, especially for the area of any peak. Baseline usually suffers from these errors, namely, high baseline drift, periodic baseline fluctuation, and spikes. Problems can take place in each component, can change the overall performance, and will consume more cost to recover the problems. HPLC analysis has gone tremendous development from manual instrumentation to the automation instrumentation. Although HPLC method development has been improved by advances in column technology and instrumentation, problems still Arises.